The correct answer is A) They may be located in cargo handling rooms. This is because the regulations for storage batteries on tank barges do not prohibit their placement in cargo handling rooms. The key requirements are that the batteries must be located in well-ventilated spaces (B), have watertight battery boxes (C), and be secured against movement (D) - but there is no requirement that they be located outside of cargo handling rooms. The other options, B, C, and D, are all correct requirements for storage batteries on tank barges, as per the relevant regulations. However, the fact that batteries may be located in cargo handling rooms makes A the only answer choice that is not a requirement.